PucI

• Description: allantoin permease
  
  

Gene name	pucI
Synonyms	ywoE
Essential	no
Product	allantoin permease
Function	purine utilization
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	53 kDa, 7.882
Gene length, protein length	1470 bp, 490 aa
Immediate neighbours	ywoF, ywoD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36470

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

transporters/ other, utilization of nucleotides, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: purine-cytosine permease family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94575

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: pucI PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)