HisS

• Description: histidyl-tRNA synthetase
  
  

Gene name	hisS
Synonyms
Essential	yes PubMed
Product	histidyl-tRNA synthetase
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	47 kDa, 5.229
Gene length, protein length	1272 bp, 424 aa
Immediate neighbours	aspS, yrzK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27560

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

translation, essential genes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1H4V (from Thermus thermophilus, 43% identity, 61% similarity) PubMed

• UniProt: O32039

• KEGG entry: [2]

• E.C. number: 6.1.1.21

Expression and regulation

• Operon: hisS-aspS

• Sigma factor:

• Regulation:
  • induced by histidine or aspartate limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)