GerQ

• Description: spore coat protein, necessary for the proper localization of CwlJ
  
  

Gene name	gerQ
Synonyms	ywdL, ipa-62r
Essential	no
Product	spore coat protein
Function	spore germination
MW, pI	20 kDa, 7.012
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	spsA, ywdK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sporulation proteins, germination

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: cross-linked by YabG and Tgl at higher temperatures that induce germination PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: inner spore coat PubMed

Database entries

• Structure:

• UniProt: P39620

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gerQ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Alicia Monroe, Peter Setlow
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ.
J Bacteriol: 2006, 188(21);7609-16
[PubMed:16936016] [WorldCat.org] [DOI] (P p)

Ritsuko Kuwana, Naoyuki Okuda, Hiromu Takamatsu, Kazuhito Watabe
Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.
J Biochem: 2006, 139(5);887-901
[PubMed:16751597] [WorldCat.org] [DOI] (P p)

Rita Zilhão, Rachele Isticato, Lígia O Martins, Leif Steil, Uwe Völker, Ezio Ricca, Charles P Moran, Adriano O Henriques
Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis.
J Bacteriol: 2005, 187(22);7753-64
[PubMed:16267299] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Peter Setlow
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores.
J Bacteriol: 2004, 186(17);5567-75
[PubMed:15317760] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Patrick Eichenberger, Christiaan van Ooij, Peter Setlow
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate.
J Bacteriol: 2003, 185(7);2315-29
[PubMed:12644503] [WorldCat.org] [DOI] (P p)