Difference between revisions of "FusA"

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= Categories containing this gene/protein =
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{{SubtiWiki category|[[translation]]}},
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{{SubtiWiki category|[[essential genes]]}},
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{{SubtiWiki category|[[phosphoproteins]]}}
 
=The protein=
 
=The protein=
  

Revision as of 16:51, 30 November 2010

  • Description: elongation factor G

Gene name fusA
Synonyms fus
Essential yes PubMed
Product elongation factor G
Function translation
MW, pI 76 kDa, 4.615
Gene length, protein length 2076 bp, 692 aa
Immediate neighbours rpsG, tufA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FusA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU01120

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

Categories containing this gene/protein

translation, essential genes, phosphoproteins

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: hydrolyses GTP
  • Protein family: EF-G/EF-2 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) PubMed, PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1ELO (from Thermus thermophilus) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP840, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP848, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References