FosB

• Description: bacillithiol-S-transferase; confers resistence against antimicrobial compounds from B. amyloliquefaciens
  
  

Gene name	fosB
Synonyms	yndN
Essential	no
Product	fosfomycin resistance protein
Function	confers resistence against antimicrobial compounds from B. amyloliquefaciens
MW, pI	17 kDa, 6.137
Gene length, protein length	432 bp, 144 aa
Immediate neighbours	yndM, lexA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU17840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

cell envelope stress proteins (controlled by SigM, W, X, Y), resistance against toxins/ antibiotics

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: FosB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31817

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: fosB PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • expressed under stress conditions (SigW) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

John D Helmann
Bacillithiol, a new player in bacterial redox homeostasis.
Antioxid Redox Signal: 2011, 15(1);123-33
[PubMed:20712413] [WorldCat.org] [DOI] (I p)

Bronwyn G Butcher, John D Helmann
Identification of Bacillus subtilis sigma-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli.
Mol Microbiol: 2006, 60(3);765-82
[PubMed:16629676] [WorldCat.org] [DOI] (P p)

M Cao, B A Bernat, Z Wang, R N Armstrong, J D Helmann
FosB, a cysteine-dependent fosfomycin resistance protein under the control of sigma(W), an extracytoplasmic-function sigma factor in Bacillus subtilis.
J Bacteriol: 2001, 183(7);2380-3
[PubMed:11244082] [WorldCat.org] [DOI] (P p)