CysI

• Description: sulfite reductase (NADPH2)
  
  

Gene name	cysI
Synonyms	yvgR
Essential	no
Product	sulfite reductase (NADPH2)
Function	sulfite reduction
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	67 kDa, 4.573
Gene length, protein length	1815 bp, 605 aa
Immediate neighbours	cysJ, helD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33440

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sulfur metabolism

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32213

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cysI-cysJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • CysL: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

J R van der Ploeg, M Barone, T Leisinger
Functional analysis of the Bacillus subtilis cysK and cysJI genes.
FEMS Microbiol Lett: 2001, 201(1);29-35
[PubMed:11445163] [WorldCat.org] [DOI] (P p)