Difference between revisions of "BshA"
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| + | = Categories containing this gene/protein = | ||
| + | {{SubtiWiki category|[[miscellaneous metabolic pathways]]}}, | ||
| + | {{SubtiWiki category|[[resistance against oxidative and electrophile stress]]}} | ||
=The protein= | =The protein= | ||
Revision as of 15:31, 30 November 2010
- Description: L-malic acid glycosyltransferase, involved in bacillithiol synthesis
| Gene name | bshA |
| Synonyms | jojH, ypjH |
| Essential | no |
| Product | L-malic acid glycosyltransferase |
| Function | biosynthesis of bacillithiol |
| MW, pI | 41 kDa, 6.149 |
| Gene length, protein length | 1131 bp, 377 aa |
| Immediate neighbours | cca, bshB1 |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU22460
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
Categories containing this gene/protein
miscellaneous metabolic pathways, resistance against oxidative and electrophile stress
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: UDP-GlcNAc + L-malate = GlcNAc(α1→2)L-malate PubMed; also uses D-malate as a substrate, but with much lower affinity PubMed
- Protein family: NamA subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P42982
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: bshA::mls available in John_Helmann lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541]
[WorldCat.org]
[DOI]
(I p)
Gerald L Newton, Mamta Rawat, James J La Clair, Vishnu Karthik Jothivasan, Tanya Budiarto, Chris J Hamilton, Al Claiborne, John D Helmann, Robert C Fahey
Bacillithiol is an antioxidant thiol produced in Bacilli.
Nat Chem Biol: 2009, 5(9);625-7
[PubMed:19578333]
[WorldCat.org]
[DOI]
(I p)
