Difference between revisions of "GlnA"

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* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamine synthetase and effector of [[TnrA]] and [[GlnR]] <br/><br/>
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=[http://ycybesav.co.cc UNDER COSTRUCTION, PLEASE SEE THIS POST IN RESERVE COPY]=
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=[http://ycybesav.co.cc CLICK HERE]=
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* '''Description:''' [[trigger enzymes|trigger enzyme]]: glutamine synthetase and effector of [[TnrA]] and [[GlnR]] &lt;br/>&lt;br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"|'''Function''' || glutamine biosynthesis, control of [[TnrA]] and [[GlnR]] activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || glutamine biosynthesis, control of [[TnrA]] and [[GlnR]] activity
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &lt;br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 4.874   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 4.874   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glnR]]'', ''[[ynxB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glnR]]'', ''[[ynxB]]''
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13630&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;#91;EMBLCDS:CAB13630&amp;#93;+-newId sequences] &lt;br/> (Barbe ''et al.'', 2009)'''
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:glnA_context.gif]]
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|colspan="2" | '''Genetic context''' &lt;br/> [[Image:glnA_context.gif]]
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  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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  &lt;div align="right"> &lt;small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&lt;/small>&lt;/div>
 
|-
 
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|}
 
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__TOC__
 
__TOC__
  
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<br/><br/><br/><br/><br/><br/>
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&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>
  
 
=The gene=
 
=The gene=
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
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* '''Catalyzed reaction/ biological activity:''' ATP + L-glutamate + NH<sub>3</sub> = ADP + phosphate + L-glutamine (according to Swiss-Prot)  
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* '''Catalyzed reaction/ biological activity:''' ATP + L-glutamate + NH&lt;sub>3&lt;/sub> = ADP + phosphate + L-glutamine (according to Swiss-Prot)  
  
 
* '''Protein family:''' glutamine synthetase family (according to Swiss-Prot)
 
* '''Protein family:''' glutamine synthetase family (according to Swiss-Prot)
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=References=
 
=References=
 
==Reviews==
 
==Reviews==
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<pubmed> 10231480 18086213 </pubmed>
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&lt;pubmed> 10231480 18086213 &lt;/pubmed>
 
==Original publications==
 
==Original publications==
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<pubmed>19233925, 20389117,8799114,18195355, 11719184, 12139611, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156 2906311 20656908 16055443 18331450 16547045 8093698 </pubmed>
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&lt;pubmed>19233925, 20389117,8799114,18195355, 11719184, 12139611, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156 2906311 20656908 16055443 18331450 16547045 8093698 &lt;/pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 00:07, 24 November 2010

UNDER COSTRUCTION, PLEASE SEE THIS POST IN RESERVE COPY

CLICK HERE

Gene name glnA
Synonyms
Essential no
Product trigger enzyme: glutamine synthetase
Function glutamine biosynthesis, control of TnrA and GlnR activity
Metabolic function and regulation of this protein in SubtiPathways: <br/>Ammonium/ glutamate
MW, pI 50 kDa, 4.874
Gene length, protein length 1332 bp, 444 aa
Immediate neighbours glnR, ynxB
Get the DNA and protein sequences <br/> (Barbe et al., 2009)
Genetic context <br/> GlnA context.gif 
<div align="right"> <small>This image was kindly provided by SubtiList</small></div>

<br/><br/><br/><br/><br/><br/>

The gene

Basic information

  • Locus tag: BSU17460

Phenotypes of a mutant

auxotrophic for glutamine

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamate + NH<sub>3</sub> = ADP + phosphate + L-glutamine (according to Swiss-Prot)
  • Protein family: glutamine synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: K(M) for: Glu: 27 mM, ATP: 2.4 mM, ammonium: 0.18 mM; v(max): 3.7 µmol/min/mg
  • Domains: glutamate binding flap (aa 300 ... 306: protects unstable intermediates from abberant hydrolysis)
  • Modification: phosphorylated on ser/ thr/ tyr PubMed, in vitro phosphorylated by PrkC on Thr-26, Thr-147, Ser-207, and Thr-286 PubMed
  • Cofactor(s): Mg(2+)
  • Effectors of protein activity: feedback inhibition by glutamine, glutamine binds the entrance site for glutamate
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 2GLS (enzyme from Salmonella typhimurium)
  • KEGG entry: [3]

Additional information

GlnA is a homooligomer of 12 subunits

Expression and regulation

  • Regulation:
    • expressed in the absence of glutamine (GlnR) PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Additional information:

Biological materials

  • Mutant: GP247 (cat), available in Stülke lab
  • Expression vector:
    • expression/ purification from E. coli, with N-terminal Strep-tag (in pGP172): pGP174, available in Stülke lab
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

Your additional remarks

References

Reviews

<pubmed> 10231480 18086213 </pubmed>

Original publications

<pubmed>19233925, 20389117,8799114,18195355, 11719184, 12139611, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156 2906311 20656908 16055443 18331450 16547045 8093698 </pubmed>

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