Difference between revisions of "GltA"

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* '''Description:''' large subunit of glutamate synthase  <br/><br/>
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* '''Description:''' large subunit of glutamate synthase  &lt;br/>&lt;br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"|'''Function''' || glutamate biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || glutamate biosynthesis
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &lt;br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 168 kDa, 5.47  
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 168 kDa, 5.47  
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gltB]]'', ''[[gltC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gltB]]'', ''[[gltC]]''
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13728&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;#91;EMBLCDS:CAB13728&amp;#93;+-newId sequences] &lt;br/> (Barbe ''et al.'', 2009)'''
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:gltA_context.gif]]
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|colspan="2" | '''Genetic context''' &lt;br/> [[Image:gltA_context.gif]]
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  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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  &lt;div align="right"> &lt;small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&lt;/small>&lt;/div>
 
|-
 
|-
 
|}
 
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__TOC__
 
__TOC__
  
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<br/><br/><br/><br/><br/><br/>
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&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>
  
  
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
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* '''Catalyzed reaction/ biological activity:''' 2 L-glutamate + NADP<sup>+</sup> = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
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* '''Catalyzed reaction/ biological activity:''' 2 L-glutamate + NADP&lt;sup>+&lt;/sup> = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) &lt;=> L-glutamine + 2-oxoglutarate + NADPH
  
 
* '''Protein family:''' glutamate synthase family (according to Swiss-Prot) glutamate synthase family
 
* '''Protein family:''' glutamate synthase family (according to Swiss-Prot) glutamate synthase family
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** [[GltA]]-[[Icd]]  {{PubMed|20933603}}
 
** [[GltA]]-[[Icd]]  {{PubMed|20933603}}
  
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* '''Localization:''' membrane associated [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+18763711 PubMed], cytoplasm
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* '''Localization:''' membrane associated [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Abstract&amp;list_uids=+18763711 PubMed], cytoplasm
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
  
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subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Abstract&amp;list_uids=+17981983 PubMed]
  
 
=Expression and regulation=
 
=Expression and regulation=
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=References=
 
=References=
  
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<pubmed>12823818,,11029411,12850135 7559360 15150225 2548995 17183217, 17608797,17134717,14523131,12823818, 18326565,17981983,18763711 20933603 17012385 </pubmed>
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&lt;pubmed>12823818,,11029411,12850135 7559360 15150225 2548995 17183217, 17608797,17134717,14523131,12823818, 18326565,17981983,18763711 20933603 17012385 &lt;/pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 23:19, 23 November 2010

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  • Description: large subunit of glutamate synthase <br/><br/>
Gene name gltA
Synonyms
Essential no
Product glutamate synthase (large subunit)
Function glutamate biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: <br/>Ammonium/ glutamate
MW, pI 168 kDa, 5.47
Gene length, protein length 4560 bp, 1520 amino acids
Immediate neighbours gltB, gltC
Get the DNA and protein sequences <br/> (Barbe et al., 2009)
Genetic context <br/> GltA context.gif 
<div align="right"> <small>This image was kindly provided by SubtiList</small></div>

<br/><br/><br/><br/><br/><br/>


The gene

Basic information

  • Locus tag: BSU18450

Phenotypes of a mutant

auxotrophic for glutamate

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 L-glutamate + NADP<sup>+</sup> = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
  • Protein family: glutamate synthase family (according to Swiss-Prot) glutamate synthase family
  • Paralogous protein(s): YerD

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Glutamine amidotransferase type-2 domain (22-415)
    • Nucleotide binding domain (1060-1112)
  • Modification:
  • Cofactor(s): 3Fe-4S, FAD, FMN
  • Effectors of protein activity:
  • Localization: membrane associated PubMed, cytoplasm

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • expression activated by glucose (11 fold) (CcpA, GltC) PubMed
    • repressed by arginine (GltC, RocG) PubMed
    • expressed in the presence of ammonium PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant: GP807 (del gltAB::tet), GP222 (gltA under the control of p-xyl), available in Stülke lab
  • Expression vector:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

<pubmed>12823818,,11029411,12850135 7559360 15150225 2548995 17183217, 17608797,17134717,14523131,12823818, 18326565,17981983,18763711 20933603 17012385 </pubmed>

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