Difference between revisions of "GltX"
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* '''Interactions:''' | * '''Interactions:''' | ||
| + | ** ** ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]] (the "transamidosome") {{PubMed|20882017}} | ||
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* '''Localization:''' cytoplasm (according to Swiss-Prot) | * '''Localization:''' cytoplasm (according to Swiss-Prot) | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId= | + | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3AKZ 3AKZ] (complex with tRNA-Gln from ''Thermotoga maritima'', 38% identity, 62% similarity) {{PubMed|20882017}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P22250 P22250] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P22250 P22250] | ||
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=References= | =References= | ||
| − | <pubmed>12884008,, </pubmed> | + | <pubmed>12884008,20882017, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:40, 14 October 2010
- Description: glutamyl-tRNA synthetase, universally conserved protein
| Gene name | gltX |
| Synonyms | |
| Essential | yes PubMed |
| Product | glutamyl-tRNA synthetase |
| Function | translation |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Cys, Met & Sulfate assimilation | |
| MW, pI | 55 kDa, 5.021 |
| Gene length, protein length | 1449 bp, 483 aa |
| Immediate neighbours | ispF, cysE |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU00920
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 3AKZ (complex with tRNA-Gln from Thermotoga maritima, 38% identity, 62% similarity) PubMed
- UniProt: P22250
- KEGG entry: [3]
- E.C. number: 6.1.1.17
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
