AroE

• Description: 3-phosphoshikimate 1-carboxyvinyltransferase
  
  

Gene name	aroE
Synonyms
Essential	no
Product	3-phosphoshikimate 1-carboxyvinyltransferase
Function	biosynthesis of aromatic amino acids
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	45 kDa, 6.341
Gene length, protein length	1284 bp, 428 aa
Immediate neighbours	ypiA, tyrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU22600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate (according to Swiss-Prot)

• Protein family: ABC transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1RF5 (from Streptococcus pneumoniae, 52% identity, 69% similarity) PubMed

• UniProt: P20691

• KEGG entry: [3]

• E.C. number: 2.5.1.19

Expression and regulation

• Operon:
  • aroF-aroB-aroH-trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE (according to DBTBS)
  • trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE PubMed
  • hisC-tyrA-aroE (according to DBTBS)

• Sigma factor:
  • trpE: SigA PubMed

• Regulation:
  • induction by tryptophan ( TRAP) PubMed

• Regulatory mechanism:
  • TRAP: binding to the mRNA in the presence of tryptophan, this results in transcription termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

D J Henner, L Band, G Flaggs, E Chen
The organization and nucleotide sequence of the Bacillus subtilis hisH, tyrA and aroE genes.
Gene: 1986, 49(1);147-52
[PubMed:3106153] [WorldCat.org] [DOI] (P p)