Difference between revisions of "GlmM"
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3I3W 3I3W] (from ''Francisella tularensis'', 39% identity, 58% similarity) |
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34824 O34824] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O34824 O34824] | ||
Revision as of 13:41, 30 July 2010
- Description: phosphoglucosamine mutase, required for cell wall synthesis
| Gene name | glmM |
| Synonyms | ybbT |
| Essential | yes PubMed |
| Product | phosphoglucosamine mutase, required for cell wall synthesis |
| Function | cell wall synthesis |
| MW, pI | 48 kDa, 4.677 |
| Gene length, protein length | 1344 bp, 448 aa |
| Immediate neighbours | ybbR, glmS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU01770
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)
- Protein family: phosphohexose mutase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 3I3W (from Francisella tularensis, 39% identity, 58% similarity)
- UniProt: O34824
- KEGG entry: [2]
- E.C. number: 5.4.2.10
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- pGP400: (expression in B. subtilis in pBQ200), available in Stülke lab
- pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Stülke lab
- pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Stülke lab
- pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Stülke lab
- pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
