GlpX

• Description: class II fructose-1,6-bisphosphatase, gluconeogenesis
  
  

Gene name	glpX
Synonyms	ywjI
Essential	no
Product	class II fructose-1,6-bisphosphatase
Function	gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	33 kDa, 5.067
Gene length, protein length	963 bp, 321 aa
Immediate neighbours	rho, murAB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate ==> D-fructose 6-phosphate + Pi

• Protein family: FBPase class 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(M) for FBP: 20 µM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3D1R (from E. coli, 49% identity, 68% similarity)PubMed

• UniProt: Q03224

• KEGG entry: [3]

• E.C. number: 3.1.3.11

Expression and regulation

• Operon: murAB-glpX PubMed

• Sigma factor:

• Regulation: constitutive PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Matthieu Jules, Ludovic Le Chat, Stéphane Aymerich, Dominique Le Coq
The Bacillus subtilis ywjI (glpX) gene encodes a class II fructose-1,6-bisphosphatase, functionally equivalent to the class III Fbp enzyme.
J Bacteriol: 2009, 191(9);3168-71
[PubMed:19270101] [WorldCat.org] [DOI] (I p)

Greg Brown, Alexander Singer, Vladimir V Lunin, Michael Proudfoot, Tatiana Skarina, Robert Flick, Samvel Kochinyan, Ruslan Sanishvili, Andrzej Joachimiak, Aled M Edwards, Alexei Savchenko, Alexander F Yakunin
Structural and biochemical characterization of the type II fructose-1,6-bisphosphatase GlpX from Escherichia coli.
J Biol Chem: 2009, 284(6);3784-92
[PubMed:19073594] [WorldCat.org] [DOI] (P p)