Difference between revisions of "QueF"
| Line 93: | Line 93: | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** repressed in the presence of queuosine ([[preQ1 riboswitch]]) {{PubMed|19285444}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[preQ1 riboswitch]]: transcriptional antitermination in the absence of queuosine {{PubMed|19285444}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 118: | Line 120: | ||
=References= | =References= | ||
| − | <pubmed>14660578,15767583 16511203 ,17929836 | + | <pubmed>14660578,15767583 16511203 ,17929836 19285444 17384645 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:54, 17 June 2010
- Description: nitrile reductase, synthesis of the modified ribonucleotide queuosine
| Gene name | queF |
| Synonyms | ykvM |
| Essential | no |
| Product | nitrile reductase |
| Function | tRNA modification |
| MW, pI | 19 kDa, 4.927 |
| Gene length, protein length | 495 bp, 165 aa |
| Immediate neighbours | queE, ykvN |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU13750
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH (according to Swiss-Prot)
- Protein family: QueF type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31678
- KEGG entry: [2]
- E.C. number: 1.7.1.13
Additional information
Expression and regulation
- Regulation:
- repressed in the presence of queuosine (preQ1 riboswitch) PubMed
- Regulatory mechanism:
- preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
