Rex

• Description: transcriptional repressor of anaerobically expressed genes involved in anaerobic respiration and fermentation
  
  

Gene name	rex
Synonyms	ydiH
Essential	no
Product	transcriptional repressor
Function	regulation of fermentation and anaerobic respiration
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	23 kDa, 6.772
Gene length, protein length	645 bp, 215 aa
Immediate neighbours	ydiG, tatAY
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU05970

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: transcriptional regulatory rex family (according to Swiss-Prot)

• Paralogous protein(s):

Genes/ operons controlled by Rex

alsS-alsD, cydA-cydB-cydC-cydD, ldh-lctP, ywcJ PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: NADH (indication of low oxygen concentration) releases Rex from DNA PubMed, Rex has a very highh affinity for NADH

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2VT2, 2VT3

• UniProt: O05521

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

• Mutant: LUW292 (erm), GP834 (erm), both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Claes von Wachenfeldt, Lund University, Sweden Homepage