Difference between revisions of "Hag"
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** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12730172 PubMed] | ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12730172 PubMed] | ||
** [[CsrA]]: translation inhibition {{PubMed|17555441}} | ** [[CsrA]]: translation inhibition {{PubMed|17555441}} | ||
| + | ** [[ScoC]]: transcription repression {{PubMed|19118355}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| − | <pubmed>2498284,9648743,9096206,7708689,19202088 | + | <pubmed>2498284,9648743,9096206,7708689,19202088, 18763711, 19118355 17555441 10809682 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 18:49, 14 May 2010
- Description: flagellin protein
| Gene name | hag |
| Synonyms | |
| Essential | no |
| Product | flagellin protein |
| Function | movement and chemotaxis |
| MW, pI | 32 kDa, 4.782 |
| Gene length, protein length | 912 bp, 304 aa |
| Immediate neighbours | yvyC, csrA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU35360
Phenotypes of a mutant
No swarming motility on B medium. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: bacterial flagellin family (according to Swiss-Prot)
- Paralogous protein(s): YvzB (C-terminal domain of Hag)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular (no signal peptide), major component of the secretome PubMed, membrane PubMed
Database entries
- Structure:
- UniProt: P02968
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab
- Expression vector:
- lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088]
[WorldCat.org]
[DOI]
(P p)
Prashant Kodgire, K Krishnamurthy Rao
hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC.
Microbiology (Reading): 2009, 155(Pt 1);142-149
[PubMed:19118355]
[WorldCat.org]
[DOI]
(P p)
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)
Helen Yakhnin, Pallavi Pandit, Tom J Petty, Carol S Baker, Tony Romeo, Paul Babitzke
CsrA of Bacillus subtilis regulates translation initiation of the gene encoding the flagellin protein (hag) by blocking ribosome binding.
Mol Microbiol: 2007, 64(6);1605-20
[PubMed:17555441]
[WorldCat.org]
[DOI]
(P p)
D B Mirel, W F Estacio, M Mathieu, E Olmsted, J Ramirez, L M Márquez-Magaña
Environmental regulation of Bacillus subtilis sigma(D)-dependent gene expression.
J Bacteriol: 2000, 182(11);3055-62
[PubMed:10809682]
[WorldCat.org]
[DOI]
(P p)
T Caramori, A Galizzi
The UP element of the promoter for the flagellin gene, hag, stimulates transcription from both SigD- and SigA-dependent promoters in Bacillus subtilis.
Mol Gen Genet: 1998, 258(4);385-8
[PubMed:9648743]
[WorldCat.org]
[DOI]
(P p)
Y F Chen, J D Helmann
DNA-melting at the Bacillus subtilis flagellin promoter nucleates near -10 and expands unidirectionally.
J Mol Biol: 1997, 267(1);47-59
[PubMed:9096206]
[WorldCat.org]
[DOI]
(P p)
K Fredrick, T Caramori, Y F Chen, A Galizzi, J D Helmann
Promoter architecture in the flagellar regulon of Bacillus subtilis: high-level expression of flagellin by the sigma D RNA polymerase requires an upstream promoter element.
Proc Natl Acad Sci U S A: 1995, 92(7);2582-6
[PubMed:7708689]
[WorldCat.org]
[DOI]
(P p)
D B Mirel, M J Chamberlin
The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase.
J Bacteriol: 1989, 171(6);3095-101
[PubMed:2498284]
[WorldCat.org]
[DOI]
(P p)
