Difference between revisions of "Pgk"
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | ||
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− | |style="background:#ABCDEF;" align="center"| '''Essential''' || | + | |style="background:#ABCDEF;" align="center"| '''Essential''' || No |
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|style="background:#ABCDEF;" align="center"| '''Product''' || phosphoglycerate kinase | |style="background:#ABCDEF;" align="center"| '''Product''' || phosphoglycerate kinase |
Revision as of 08:57, 23 March 2010
- Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, universally conserved protein
Gene name | pgk |
Synonyms | |
Essential | No |
Product | phosphoglycerate kinase |
Function | enzyme in glycolysis/ gluconeogenesis |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 42,0 kDa, 4.77 |
Gene length, protein length | 1182 bp, 394 amino acids |
Immediate neighbours | tpi, gapA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU33930
Phenotypes of a mutant
- no growth on LB medium, the mutant requires glucise and malate for growth
- essential acoording to Kobayashi et al. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)
- Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed
- Domains:
- nucleotide binding domain (ATP) (350–353)
- 2x substrate binding domain (21–23), (59–62)
- Cofactor(s): Mg2+ or Mn2+ PubMed
- Effectors of protein activity:
- Inhibited by Co2+, NDP and NMP PubMed
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed
Database entries
- UniProt: P40924
- KEGG entry: [3]
- E.C. number: 2.7.2.3
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Expression vector:
- pGP1102 (N-terminal His-tag, in pWH844), available in Stülke lab
- pGP95 (N-terminal Strep-tag, in pGP172), available in Stülke lab
- pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
- pGP1513 (expression in B. subtilis, in pBQ200), available in Stülke lab
- lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Stülke lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References