Difference between revisions of "PBQ200"
| Line 3: | Line 3: | ||
'''Application:''' | '''Application:''' | ||
| − | The vector was constructed in the George Rapoport lab and it is suitable for constitutive overexpression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pBQ200 is based on the vector pHT315. | + | The vector was constructed in the George Rapoport lab and it is suitable for constitutive overexpression of proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pBQ200 is based on the vector pHT315. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR. |
| − | |||
| + | Sequencing primers: | ||
| + | *'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘ | ||
| + | *'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘ | ||
'''Reference:''' | '''Reference:''' | ||
<pubmed> 8057358 </pubmed> | <pubmed> 8057358 </pubmed> | ||
Revision as of 11:51, 25 February 2010
Application:
The vector was constructed in the George Rapoport lab and it is suitable for constitutive overexpression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pBQ200 is based on the vector pHT315. A Shine-Dalgarno sequence has to be fused to the open reading frame during PCR.
Sequencing primers:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
Reference:
I Martin-Verstraete, M Débarbouillé, A Klier, G Rapoport
Interactions of wild-type and truncated LevR of Bacillus subtilis with the upstream activating sequence of the levanase operon.
J Mol Biol: 1994, 241(2);178-92
[PubMed:8057358]
[WorldCat.org]
[DOI]
(P p)
