ThiM

• Description: hydroxyethylthiazole kinase
  
  

Gene name	thiM
Synonyms	thiK, ipa-25d, ywbJ
Essential	no
Product	hydroxyethylthiazole kinase
Function	biosynthesis of thiamine
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	28 kDa, 5.393
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	thiE, ywbI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)

• Protein family: Thz kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ThiM-ThiM PubMed

• Localization:

Database entries

• Structure: 1C3Q

• UniProt: P39593

• KEGG entry: [3]

• E.C. number: 2.7.1.50

Expression and regulation

• Operon: ywbI-thiM-thiE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

N Campobasso, I I Mathews, T P Begley, S E Ealick
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Biochemistry: 2000, 39(27);7868-77
[PubMed:10891066] [WorldCat.org] [DOI] (P p)

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)