Difference between revisions of "ThiM"
| Line 13: | Line 13: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine | ||
| − | |||
| − | |||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 5.393 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 5.393 | ||
Revision as of 10:41, 16 February 2010
- Description: hydroxyethylthiazole kinase
| Gene name | thiM |
| Synonyms | thiK, ipa-25d, ywbJ |
| Essential | no |
| Product | hydroxyethylthiazole kinase |
| Function | biosynthesis of thiamine |
| MW, pI | 28 kDa, 5.393 |
| Gene length, protein length | 816 bp, 272 aa |
| Immediate neighbours | thiE, ywbI |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU38300
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)
- Protein family: Thz kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1C3Q
- UniProt: P39593
- KEGG entry: [3]
- E.C. number: 2.7.1.50
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578]
[WorldCat.org]
[DOI]
(I p)
Original publications
N Campobasso, I I Mathews, T P Begley, S E Ealick
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Biochemistry: 2000, 39(27);7868-77
[PubMed:10891066]
[WorldCat.org]
[DOI]
(P p)
Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923]
[WorldCat.org]
[DOI]
(P p)
