Difference between revisions of "PhrG"
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=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[rapG]]-[[phrG]]'' {{PubMed|12950930}} | ||
| − | * ''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16553878}}, SigH {{PubMed|12950930}} |
| − | * '''[[ | + | * '''Regulation:''' |
| + | ** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed] | ||
| + | ** repressed by [[RghR]] {{PubMed|16553878}} | ||
| + | ** repressed by glucose (4.8-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | ||
| + | |||
| + | * '''Regulatory mechanism:''' | ||
| + | ** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed] | ||
| + | ** [[RghR]]: transcription repression {{PubMed|16553878}} | ||
| + | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | ||
| − | + | * '''Additional information:''' | |
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | * '''Additional information:''' | ||
=Biological materials = | =Biological materials = | ||
Revision as of 20:26, 11 February 2010
- Description: response regulator aspartate phosphatase (RapG) regulator
| Gene name | phrG |
| Synonyms | yycL |
| Essential | no |
| Product | phosphatase (RapG) regulator |
| Function | control of DegU activity |
| MW, pI | 4 kDa, 9.017 |
| Gene length, protein length | 114 bp, 38 aa |
| Immediate neighbours | rapG, rocF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU40310
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binds to RapG, this results in the inability of RapG to interact with DegU PubMed
- Protein family: phr family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: O32295
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor: SigA PubMed, SigH PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Kentaro Hayashi, Tsukahara Kensuke, Kazuo Kobayashi, Naotake Ogasawara, Mitsuo Ogura
Bacillus subtilis RghR (YvaN) represses rapG and rapH, which encode inhibitors of expression of the srfA operon.
Mol Microbiol: 2006, 59(6);1714-29
[PubMed:16553878]
[WorldCat.org]
[DOI]
(P p)
Mitsuo Ogura, Kana Shimane, Kei Asai, Naotake Ogasawara, Teruo Tanaka
Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1685-97
[PubMed:12950930]
[WorldCat.org]
[DOI]
(P p)
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)
