Difference between revisions of "SacY"
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=Expression and regulation= | =Expression and regulation= | ||
+ | * '''Operon:''' ''[[sacX]]-[[sacY]]'' {{PubMed|2116367}} | ||
− | * ''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1400159}} |
− | * ''' | + | * '''Regulation:''' induction by sucrose (at high concentration) {{PubMed|8535520}} |
− | * ''' | + | * '''Regulatory mechanism:''' |
− | + | ** induction by [[SacY]]-dependent [[RNA switch]] (transcriptional antitermination) {{PubMed|8535520}} | |
− | * | + | ** [[DegU]]-P: transcription activation {{PubMed|1400159}} |
* '''Additional information:''' | * '''Additional information:''' | ||
Line 124: | Line 125: | ||
=References= | =References= | ||
− | <pubmed>2105292 8702561 11580842 1279678 9305643 9305644 9202047 10610766, </pubmed> | + | <pubmed>2105292 8702561 11580842 1279678 9305643 9305644 9202047 10610766,2116367 1400159 8535520 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 21:00, 4 February 2010
- Description: transcriptional antiterminator for sacB and sacX-sacY (acts at high sucrose concentrations)
Gene name | sacY |
Synonyms | ipa-13r, sacS |
Essential | no |
Product | transcriptional antiterminator |
Function | regulation of sucrose utilization |
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Stress | |
MW, pI | 32 kDa, 5.883 |
Gene length, protein length | 840 bp, 280 aa |
Immediate neighbours | sacX, gspA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU38420
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binding to the mRNA of sacB and the sacX-sacY operon, causes transcription antitermination (in presence of sucrose)
- Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1AUU (RNA-binding domain)
- UniProt: P15401
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: induction by sucrose (at high concentration) PubMed
- Regulatory mechanism:
- induction by SacY-dependent RNA switch (transcriptional antitermination) PubMed
- DegU-P: transcription activation PubMed
- Additional information:
Biological materials
- Mutant: GP425 (cat), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References