Difference between revisions of "NarI"
| Line 87: | Line 87: | ||
=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[narG]]-[[narH]]-[[narJ]]-[[narI]]'' {{PubMed|8846791,16428414}} | ||
| − | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8846791,16428414}} | |
| − | |||
| − | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** expressed under anaerobic conditions ([[Fnr]]) | + | ** expressed under anaerobic conditions ([[Fnr]]) {{PubMed|8846791,16428414}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[Fnr]]: transcription activation | + | ** [[Fnr]]: transcription activation {{PubMed|8846791,16428414}} |
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 16:41, 28 January 2010
- Description: nitrate reductase (gamma subunit)
| Gene name | narI |
| Synonyms | |
| Essential | no |
| Product | nitrate reductase (gamma subunit) |
| Function | nitrate respiration, nitrogen assimilation |
| MW, pI | 25 kDa, 10.04 |
| Gene length, protein length | 669 bp, 223 aa |
| Immediate neighbours | ywiE, narJ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU37250
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P42177
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414]
[WorldCat.org]
[DOI]
(P p)
M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926]
[WorldCat.org]
[DOI]
(P p)
H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791]
[WorldCat.org]
[DOI]
(P p)
