FeuA

• Description: ABC transporter for the siderophores enterobactin and bacillibactin (binding protein), with YusV as ATPase
  
  

Gene name	feuA
Synonyms
Essential	no
Product	ABC transporter for the siderophores enterobactin and bacillibactin (binding protein)
Function	iron acquisition
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	34 kDa, 8.018
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	feuB, btr
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU01630

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/biological activity:

Substrate binding protein for the triscatecholate siderophores ferri-bacillibactin and ferri-enterobactin and part of the FeuABC-YusV ATP-binding cassette-type transporter. Furthermore ferric complexes of L-norepinephrine are bound by this protein.

• Protein family: Fe/B12 periplasmic-binding domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), extracellular (signal peptide) PubMed, membrane PubMed

Database entries

• Structure: 2PHZ, 2WI8, 2WHY (complex with ferri-bacillibactin)

• UniProt: P40409

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: feuA-feuB-feuC-ybbA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of iron (Fur, CitB) PubMed
  • induced in the presence of iron chelators bacillibactin or enterobactin (Btr) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed
  • CitB: binding to the iron responsive element in the absence of iron
  • Btr: transcription activation in the presence of the co-activators bacillibactin or enterobactin PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage