Difference between revisions of "AraE"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''araE'' {{PubMed|9401028}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9401028}}
  
* '''Regulation:''' repressed by glucose ([[CcpA]]),  induced by arabinose [http://www.ncbi.nlm.nih.gov/sites/entrez/9620981 PubMed]
+
* '''Regulation:'''  
** repressed in the absence of arabinose ([[AraR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
+
** repressed by glucose ([[CcpA]]) {{PubMed|9401028}}
 +
** induced by arabinose ([[AraR]]) {{PubMed|9401028,9620981,10417639}}
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
+
* '''Regulatory mechanism:'''  
** [[AraR]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
+
** [[CcpA]]: transcription repression  
 +
** [[AraR]]:  transcription repression {{PubMed|9401028,9620981,10417639}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 10:58, 29 December 2009

  • Description: L-arabinose permease

Gene name araE
Synonyms yvbR
Essential no
Product L-arabinose permease
Function uptake of arabinose, galactose and xylose
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 50 kDa, 9.259
Gene length, protein length 1392 bp, 464 aa
Immediate neighbours cggR, araR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AraE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU33960

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transports arabinose, and also xylose and galactose PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

O Krispin, R Allmansberger
The Bacillus subtilis AraE protein displays a broad substrate specificity for several different sugars.
J Bacteriol: 1998, 180(12);3250-2
[PubMed:9620981] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, S S Ramos
Cloning, functional analysis, and transcriptional regulation of the Bacillus subtilis araE gene involved in L-arabinose utilization.
J Bacteriol: 1997, 179(24);7705-11
[PubMed:9401028] [WorldCat.org] [DOI] (P p)