CitG

• Description: fumarase
  
  

Gene name	citG
Synonyms
Essential	no
Product	fumarate hydratase
Function	TCA cycle
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	50 kDa, 5.475
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	yuxN, gerAA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate = fumarate + H₂O (according to Swiss-Prot)

• Protein family: Fumarase subfamily (according to Swiss-Prot)

• Paralogous protein(s): AnsB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1YFE (E.coli)

• UniProt: P07343

• KEGG entry: [3]

• E.C. number: 4.2.1.2

Expression and regulation

• Operon: citG

• Sigma factor: SigA, SigH

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP718 (spec), available in Stülke lab

• Expression vector:
  • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)

• lacZ fusion: pGP387 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody: