Difference between revisions of "LevF"

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=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed]
  
* '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]''
+
* '''[[Sigma factor]]:''' [[SigL]] {{PubMed|1924373}}
  
* '''[[Sigma factor]]:''' [[SigL]]
+
* '''Regulation:'''
 +
** subject to carbon catabolite repression ([[CcpA]], [[LevR]]) {{PubMed|7592486}}
 +
** induced in the presence of fructose ([[LevR]]) {{PubMed|1900939}}
  
* '''Regulation:''' repressed by glucose ([[CcpA]]) ,  carbon catabolite repression, induction by fructose
+
* '''Regulatory mechanism:'''  
 
+
** [[CcpA]]: transcription repression {{PubMed|7592486}}
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression,  catabolite repression: transcription repression by [[CcpA]], transcription activator [[LevR]] is less active in the presence of glucose; induction: transcription activation by [[LevR]]
+
** [[LevR]]: transcription activation {{PubMed|1900939}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>10627040 2117666, </pubmed>
+
<pubmed>10627040 2117666, 1924373 7592486 1900939</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:46, 25 November 2009

Gene name levF
Synonyms
Essential no
Product fructose-specific phosphotransferase system
EIIC component
Function fructose uptake and phosphorylation
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 27 kDa, 4.395
Gene length, protein length 807 bp, 269 aa
Immediate neighbours levG, levE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LevF context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU27050

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PTS permease, mannose permease (Man) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

I Martin-Verstraete, J Stülke, A Klier, G Rapoport
Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon.
J Bacteriol: 1995, 177(23);6919-27
[PubMed:7592486] [WorldCat.org] [DOI] (P p)

M Débarbouillé, I Martin-Verstraete, F Kunst, G Rapoport
The Bacillus subtilis sigL gene encodes an equivalent of sigma 54 from gram-negative bacteria.
Proc Natl Acad Sci U S A: 1991, 88(20);9092-6
[PubMed:1924373] [WorldCat.org] [DOI] (P p)

M Débarbouillé, I Martin-Verstraete, A Klier, G Rapoport
The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both sigma 54- and phosphotransferase system-dependent regulators.
Proc Natl Acad Sci U S A: 1991, 88(6);2212-6
[PubMed:1900939] [WorldCat.org] [DOI] (P p)

I Martin-Verstraete, M Débarbouillé, A Klier, G Rapoport
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
J Mol Biol: 1990, 214(3);657-71
[PubMed:2117666] [WorldCat.org] [DOI] (P p)