Difference between revisions of "NusB"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[nusB]]-[[folD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2536750 PubMed]
+
* '''Operon:''' ''[[nusB]]-[[folD]]'' {{PubMed|2536750,11591660}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** repressed in the presence of purine nucleotides ([[PurR]]) {{PubMed|2536750,11591660}}
 
** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
 
** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
** repressed in the presence of purine nucleotides ([[PurR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]
 
** repressed in the presence of adenine or adenosine([[PurR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/2536750 PubMed]
 
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[PurR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]
+
** [[PurR]]: transcription repression (molecular inducer: PRPP) {{PubMed|2536750,11591660}}
** [[PurR]]: transcription repression (molecular inducer: PRPP) [http://www.ncbi.nlm.nih.gov/sites/entrez/2536750 PubMed]
 
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 20:05, 20 November 2009

  • Description: probable transcription termination

Gene name nusB
Synonyms yqhZ
Essential no
Product unknown
Function unknown
MW, pI 14 kDa, 4.757
Gene length, protein length 393 bp, 131 aa
Immediate neighbours folD, yqhY
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NusB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU24320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nusB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
    • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)