Difference between revisions of "AnsA"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[ansA]]-[[ansB]]'' | + | * '''Operon:''' ''[[ansA]]-[[ansB]]'' {{PubMed|1711029}} |
| − | * '''Sigma factor:''' [[SigA]] | + | * '''Sigma factor:''' [[SigA]] {{PubMed|1711029}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** expressed in the presence of asparagine ([[AnsR]]) {{PubMed|11914346}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[AnsR]]: transcription repression {{PubMed|11914346}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 120: | Line 122: | ||
=References= | =References= | ||
| − | <pubmed>8478318,1711029,, | + | <pubmed>8478318,1711029,,11914346, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:03, 18 November 2009
- Description: L-asparaginase
| Gene name | ansA |
| Synonyms | |
| Essential | no |
| Product | L-asparaginase |
| Function | asparagine degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn | |
| MW, pI | 36 kDa, 4.455 |
| Gene length, protein length | 987 bp, 329 aa |
| Immediate neighbours | ansB, ansR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU23580
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
- Protein family: asparaginase 1 family (according to Swiss-Prot)
- Paralogous protein(s): AnsZ
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P26900
- KEGG entry: [3]
- E.C. number: 3.5.1.1
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346]
[WorldCat.org]
[DOI]
(P p)
D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318]
[WorldCat.org]
[DOI]
(P p)
D X Sun, P Setlow
Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.
J Bacteriol: 1991, 173(12);3831-45
[PubMed:1711029]
[WorldCat.org]
[DOI]
(P p)
