Difference between revisions of "DfrA"
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| − | * '''Operon:''' | + | * '''Operon:''' ''[[thyB]]-[[dfrA]]'' {{PubMed|2840350}} |
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* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 11:50, 1 November 2009
- Description: dihydrofolate reductase
| Gene name | dfrA |
| Synonyms | tmp |
| Essential | yes PubMed |
| Product | dihydrofolate reductase |
| Function | biosynthesis of folate |
| Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Folate | |
| MW, pI | 19 kDa, 5.219 |
| Gene length, protein length | 504 bp, 168 aa |
| Immediate neighbours | ypkP, thyB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU21810
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH (according to Swiss-Prot)
- Protein family: spmB family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1ZDR (Geobacillus stearothermophilus)
- UniProt: P11045
- KEGG entry: [2]
- E.C. number: 1.5.1.3
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
M Iwakura, M Kawata, K Tsuda, T Tanaka
Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.
Gene: 1988, 64(1);9-20
[PubMed:2840350]
[WorldCat.org]
[DOI]
(P p)
T T Myoda, V L Funanage
Coregulation of dihydrofolate reductase and thymidylate synthase B in bacillus subtilis.
Biochim Biophys Acta: 1985, 824(2);99-103
[PubMed:3918568]
[WorldCat.org]
[DOI]
(P p)
T T Myoda, S V Lowther, V L Funanage, F E Young
Cloning and mapping of the dihydrofolate reductase gene of Bacillus subtilis.
Gene: 1984, 29(1-2);135-43
[PubMed:6436144]
[WorldCat.org]
[DOI]
(P p)
