Difference between revisions of "BglS"
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* '''Operon:''' | * '''Operon:''' | ||
| − | ** ''[[licT]]-[[bglS]]'' | + | ** ''[[licT]]-[[bglS]]'' {{PubMed|8606172}} |
| − | ** ''[[bglS]]'' | + | ** ''[[bglS]]'' {{PubMed|8606172}} |
| − | * '''[[Sigma factor]]:''' [[SigA]] | + | * '''[[Sigma factor]]:''' |
| + | ** ''[[licT]]'': [[SigA]] {{PubMed|8606172}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
** repressed by glucose (3.1-fold) ([[CcpA]]) {{PubMed|12850135,8245831}} | ** repressed by glucose (3.1-fold) ([[CcpA]]) {{PubMed|12850135,8245831}} | ||
** expressed in the stationary phase (temporal activation) {{PubMed|8245830}} | ** expressed in the stationary phase (temporal activation) {{PubMed|8245830}} | ||
| − | ** weak induction by ß-glucosides ([[LicT]]) | + | ** weak induction by ß-glucosides ([[LicT]]) {{PubMed|8606172}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8245831 PubMed] | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8245831 PubMed] | ||
| − | ** [[LicT]]: binding to an [[RNA switch]] results in transcriptional antitermination | + | ** [[LicT]]: binding to an [[RNA switch]] results in transcriptional antitermination {{PubMed|8606172}} |
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 19:36, 31 October 2009
- Description: endo-beta-1,3-1,4 glucanase
| Gene name | bglS |
| Synonyms | bgl, licS |
| Essential | no |
| Product | endo-beta-1,3-1,4 glucanase |
| Function | lichenan degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 27 kDa, 6.482 |
| Gene length, protein length | 726 bp, 242 aa |
| Immediate neighbours | citH, licT |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU39070
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 16 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular (signal peptide) PubMed
Database entries
- Structure:
- UniProt: P04957
- KEGG entry: [3]
- E.C. number: 3.2.1.73 3
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- CcpA: transcription repression PubMed
- LicT: binding to an RNA switch results in transcriptional antitermination PubMed
- Additional information:
Biological materials
- Mutant: GP427 (licT-bglS, erm), BGW7 (cat), both available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
