Difference between revisions of "MleN"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
Line 106: Line 106:
 
* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:'''
+
* '''lacZ fusion:''' pGP388 (in [[pAC7]]), available in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 18:21, 29 October 2009

  • Description: malate-H+/Na+-lactate antiporter

Gene name mleN
Synonyms yqkI
Essential no
Product malate-H+/Na+-lactate antiporter
Function malate uptake
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 50 kDa, 7.249
Gene length, protein length 1404 bp, 468 aa
Immediate neighbours mleA, ansB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MleN context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU23560

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)
  • Paralogous protein(s): NhaC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: membrane associated PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP388 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References