Difference between revisions of "Phy"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''phy'' {{PubMed|7592393}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' none {{PubMed|16980498}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 97: | Line 97: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** the gene is not expressed in ''B. subtilis'' due to the absene of a functional promoter {{PubMed|16980498}} | ||
=Biological materials = | =Biological materials = | ||
| Line 118: | Line 119: | ||
=References= | =References= | ||
| − | <pubmed>18957862, 10655618 ,</pubmed> | + | <pubmed>18957862, 10655618 7592393 ,16980498 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:19, 25 October 2009
- Description: phytase
| Gene name | phy |
| Synonyms | yodV, yzxA |
| Essential | no |
| Product | 3-phytase |
| Function | untilization of inositol hexakisphosphate (phytate) |
| MW, pI | 41 kDa, 4.936 |
| Gene length, protein length | 1146 bp, 382 aa |
| Immediate neighbours | cgeB, yodU |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU19800
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to Swiss-Prot)
- Protein family: msrA Met sulfoxide reductase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular (signal peptide) PubMed
Database entries
- Structure: 1QLG (enzyme from B. amyloliquefaciens, 72% identity)
- UniProt: P42094
- KEGG entry: [3]
- E.C. number: 3.1.3.8
Additional information
Expression and regulation
- Operon: phy PubMed
- Sigma factor: none PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
- the gene is not expressed in B. subtilis due to the absene of a functional promoter PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862]
[WorldCat.org]
[DOI]
(I p)
Oliwia Makarewicz, Sarah Dubrac, Tarek Msadek, Rainer Borriss
Dual role of the PhoP approximately P response regulator: Bacillus amyloliquefaciens FZB45 phytase gene transcription is directed by positive and negative interactions with the phyC promoter.
J Bacteriol: 2006, 188(19);6953-65
[PubMed:16980498]
[WorldCat.org]
[DOI]
(P p)
N C Ha, B C Oh, S Shin, H J Kim, T K Oh, Y O Kim, K Y Choi, B H Oh
Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states.
Nat Struct Biol: 2000, 7(2);147-53
[PubMed:10655618]
[WorldCat.org]
[DOI]
(P p)
S Roels, R Losick
Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis.
J Bacteriol: 1995, 177(21);6263-75
[PubMed:7592393]
[WorldCat.org]
[DOI]
(P p)
