Difference between revisions of "Pgk"
(→Expression and regulation) |
|||
| Line 95: | Line 95: | ||
* '''Operon:''' | * '''Operon:''' | ||
| − | ** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' | + | ** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}} |
| − | ** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' | + | ** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}} |
| − | * '''Sigma factor:''' [[SigA]] | + | * '''Sigma factor:''' [[SigA]] {{PubMed|11489127}} |
| − | * '''Regulation:''' expression activated by glucose ( | + | * '''Regulation:''' |
| − | **''[[cggR]]'': induced by | + | ** expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] |
| − | **''[[pgk]]'': constitutive | + | ** ''[[cggR]]'': induced by glycolytic substrates [[CggR]] {{PubMed|11489127}} |
| + | ** ''[[pgk]]'': constitutive {{PubMed|11489127}} | ||
| − | * '''Regulatory mechanism:''' transcription repression by [[CggR]] | + | * '''Regulatory mechanism:''' transcription repression by [[CggR]] {{PubMed|11489127}} |
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 19:54, 4 October 2009
- Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme
| Gene name | pgk |
| Synonyms | |
| Essential | yes |
| Product | phosphoglycerate kinase |
| Function | enzyme in glycolysis/ gluconeogenesis |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 42,0 kDa, 4.77 |
| Gene length, protein length | 1182 bp, 394 amino acids |
| Immediate neighbours | gapA, tpi |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU33930
Phenotypes of a mutant
- Essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)
- Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed
- Domains:
- nucleotide binding domain (ATP) (350–353)
- 2x substrate binding domain (21–23), (59–62)
- Cofactor(s): Mg2+ or Mn2+ PubMed
- Effectors of protein activity:
- Inhibited by Co2+, NDP and NMP PubMed
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed
Database entries
- UniProt: P40924
- KEGG entry: [3]
- E.C. number: 2.7.2.3
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant: GP699 (cat), available in Stülke lab
- Expression vector:
- pGP1102 (N-terminal His-tag, in pWH844), available in Stülke lab
- pGP95 (N-terminal Strep-tag, in pGP172), available in Stülke lab
- pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
- pGP1513 (expression in B. subtilis, in pBQ200), available in Stülke lab
- lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Stülke lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
