Difference between revisions of "GerQ"

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* '''Description:''' spore coat protein, necessary for the proper localization of CwlJ <br/><br/>
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* '''Description:''' spore coat protein, necessary for the proper localization of [[CwlJ]] <br/><br/>
  
 
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{| align="right" border="1" cellpadding="2"  
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* '''Domains:'''  
 
* '''Domains:'''  
  
* '''Modification:'''
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* '''Modification:''' cross-linked by [[YabG]] and [[Tgl]] at higher temperatures that induce germination {{PubMed|16751597}}
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
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* '''Regulation:'''  
 
* '''Regulation:'''  
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** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|15699190}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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=References=
 
=References=
  
<pubmed>,15317760,16936016,12644503,16267299, </pubmed>
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<pubmed>,15317760,16936016,12644503,16267299, 16751597 15699190</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 06:48, 4 October 2009

  • Description: spore coat protein, necessary for the proper localization of CwlJ

Gene name gerQ
Synonyms ywdL, ipa-62r
Essential no
Product spore coat protein
Function spore germination
MW, pI 20 kDa, 7.012
Gene length, protein length 543 bp, 181 aa
Immediate neighbours spsA, ywdK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwdL context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: cross-linked by YabG and Tgl at higher temperatures that induce germination PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: spore wall (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alicia Monroe, Peter Setlow
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ.
J Bacteriol: 2006, 188(21);7609-16
[PubMed:16936016] [WorldCat.org] [DOI] (P p)

Ritsuko Kuwana, Naoyuki Okuda, Hiromu Takamatsu, Kazuhito Watabe
Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.
J Biochem: 2006, 139(5);887-901
[PubMed:16751597] [WorldCat.org] [DOI] (P p)

Rita Zilhão, Rachele Isticato, Lígia O Martins, Leif Steil, Uwe Völker, Ezio Ricca, Charles P Moran, Adriano O Henriques
Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis.
J Bacteriol: 2005, 187(22);7753-64
[PubMed:16267299] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Peter Setlow
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores.
J Bacteriol: 2004, 186(17);5567-75
[PubMed:15317760] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Patrick Eichenberger, Christiaan van Ooij, Peter Setlow
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate.
J Bacteriol: 2003, 185(7);2315-29
[PubMed:12644503] [WorldCat.org] [DOI] (P p)