Difference between revisions of "PucM"

From SubtiWiki
Jump to: navigation, search
(References)
Line 124: Line 124:
 
=References=
 
=References=
  
<pubmed>11344136,12823818,12029039,12823818,12029039, </pubmed>
+
<pubmed>11344136,12823818,12029039,16782815 , </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 06:47, 3 October 2009

  • Description: uricase

Gene name pucM
Synonyms yunM
Essential no
Product uricase
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Nucleotides (regulation)
MW, pI 13 kDa, 5.082
Gene length, protein length 363 bp, 121 aa
Immediate neighbours pucL, pucE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PucM context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU32460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate (according to Swiss-Prot)
  • Protein family: 5-hydroxyisourate hydrolase subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References