Difference between revisions of "SacY"
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| − | * '''Description:''' transcriptional antiterminator for ''[[sacB]]'' and ''[[sacX]]-[[sacY]]'' (acts at high sucrose concentrations) <br/><br/> | + | * '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] for ''[[sacB]]'' and ''[[sacX]]-[[sacY]]'' (acts at high sucrose concentrations) <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional antiterminator | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]] |
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization | ||
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* '''Catalyzed reaction/ biological activity:''' binding to the mRNA of'' [[sacB]]'' and the ''[[sacX]]-[[sacY]]'' operon, causes transcription antitermination (in presence of sucrose) | * '''Catalyzed reaction/ biological activity:''' binding to the mRNA of'' [[sacB]]'' and the ''[[sacX]]-[[sacY]]'' operon, causes transcription antitermination (in presence of sucrose) | ||
| − | * '''Protein family:''' transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators | + | * '''Protein family:''' [[PRD-containing transcription factors|transcriptional antiterminator]] bglG family (according to Swiss-Prot) BglG family of antiterminators |
* '''Paralogous protein(s):''' [[LicT]], [[GlcT]], [[SacT]] | * '''Paralogous protein(s):''' [[LicT]], [[GlcT]], [[SacT]] | ||
Revision as of 19:52, 20 September 2009
- Description: transcriptional antiterminator for sacB and sacX-sacY (acts at high sucrose concentrations)
| Gene name | sacY |
| Synonyms | ipa-13r, sacS |
| Essential | no |
| Product | transcriptional antiterminator |
| Function | regulation of sucrose utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Stress | |
| MW, pI | 32 kDa, 5.883 |
| Gene length, protein length | 840 bp, 280 aa |
| Immediate neighbours | sacX, gspA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU38420
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binding to the mRNA of sacB and the sacX-sacY operon, causes transcription antitermination (in presence of sucrose)
- Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1AUU (RNA-binding domain)
- UniProt: P15401
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: induction by sucrose (at high concentration)
- Regulatory mechanism: induction by SacY-dependent RNA switch (transcriptional antitermination)
- Additional information:
Biological materials
- Mutant: GP425 (cat), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References
