Difference between revisions of "CarA"
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' ''[[argC]]-[[argJ]]-[[argB]]-[[argD]]-[[carA]]-[[carB]]-[[argF]]'' | + | * '''Operon:''' ''[[argC]]-[[argJ]]-[[argB]]-[[argD]]-[[carA]]-[[carB]]-[[argF]]'' {{PubMed|7511775}} |
− | * '''[[Sigma factor]]:''' [[SigA]] | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7511775}} |
− | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | + | * '''Regulation:''' |
+ | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ||
+ | ** repressed by arginine ([[AhrC]]) {{PubMed|1312212}} | ||
− | * '''Regulatory mechanism:''' [[AhrC]]: transcription repression | + | * '''Regulatory mechanism:''' |
+ | ** [[AhrC]]: transcription repression {{PubMed|1312212}} | ||
* '''Additional information:''' | * '''Additional information:''' |
Revision as of 20:21, 18 September 2009
- Description: carbamoyl-phosphate transferase-arginine (subunit A)
Gene name | carA |
Synonyms | cpa |
Essential | no |
Product | carbamoyl-phosphate transferase-arginine (subunit A) |
Function | biosynthesis of arginine |
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Ammonium/ glutamate | |
MW, pI | 38 kDa, 6.212 |
Gene length, protein length | 1059 bp, 353 aa |
Immediate neighbours | argD, carB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU11230
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)
- Protein family: glutamine amidotransferase type-1 domain (according to Swiss-Prot)
- Paralogous protein(s): PyrAA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P36838
- KEGG entry: [3]
- E.C. number: 6.3.5.5
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675]
[WorldCat.org]
[DOI]
(P p)