Difference between revisions of "CsrA"
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=Biological materials = | =Biological materials = | ||
− | * '''Mutant:''' | + | * '''Mutant:''' GP469 (spc), available in [[Stülke]] lab |
− | * '''Expression vector:''' pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | + | * '''Expression vector:''' |
− | + | ** pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | |
+ | ** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP383, available in [[Stülke]] lab | ||
+ | |||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
Revision as of 17:21, 2 September 2009
- Description: similar to E. coli carbon storage regulator
Gene name | csrA |
Synonyms | yviG |
Essential | no |
Product | unknown |
Function | unknown |
MW, pI | 8 kDa, 6.09 |
Gene length, protein length | 222 bp, 74 aa |
Immediate neighbours | hag, fliW |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU35370
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: csrA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1T3O
- UniProt: P33911
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP469 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References