Difference between revisions of "LicT"
(→Biological materials) |
|||
Line 114: | Line 114: | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
+ | ** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP165, available in [[Stülke]] lab | ||
+ | ** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP315, available in [[Stülke]] lab | ||
+ | ** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP572, available in [[Stülke]] lab | ||
+ | |||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
Revision as of 17:00, 2 September 2009
- Description: transcriptional antiterminator of the bglPH operon
Gene name | licT |
Synonyms | |
Essential | no |
Product | transcriptional antiterminator (BglG family) |
Function | required for substrate-dependent induction of bglPH |
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
MW, pI | 32 kDa, 5.944 |
Gene length, protein length | 831 bp, 277 aa |
Immediate neighbours | bglS, yxiP |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU39080
Phenotypes of a mutant
no expression of the bglP-bglH operon
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binding to the mRNAs of bglS and the bglP-bglH operon, causes transcription antitermination (in presence of salicin and absence of glucose)
- Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators
Extended information on the protein
- Kinetic information:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- UniProt: P39805
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP165, available in Stülke lab
- for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP315, available in Stülke lab
- for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP572, available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Josef Deutscher, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Michael Hecker, Greifswald, Germany Homepage
Your additional remarks
References
Original description
Control of LicT activity
Structural analysis of LicT
LicT-RNA interaction