Difference between revisions of "PtsG"
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* '''Mutant:''' GP474 (cat), QB5436 (spc), QB5445 (erm), available in [[Stülke]] lab | * '''Mutant:''' GP474 (cat), QB5436 (spc), QB5445 (erm), available in [[Stülke]] lab | ||
− | * '''Expression vector:''' pGP123 (domains BA, in [[pWH844]]), | + | * '''Expression vector:''' |
+ | ** pGP123 (domains BA, in [[pWH844]]), available in [[Stülke]] lab | ||
+ | ** pGP141 (domains BA, mut: H620D, in [[pWH844]]), available in [[Stülke]] lab | ||
+ | ** pGP428 (EIIB, in [[pWH844]]), available in [[Stülke]] lab | ||
+ | ** pGP437(EIIA in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab | ||
− | * '''lacZ fusion:''' pGP34 ([[pAC5]]), pGP66 ([[pAC7]]), pGP606 (mutant terminator, [[pAC6]]), pGP532 ([[pAC7]]), series of promoter deletions are available in [[pAC5]] and [[pAC6]], series of RAT mutants are available in [[pAC6]], available in [[Stülke]] lab | + | * '''lacZ fusion:''' |
+ | ** pGP34 ([[pAC5]]), available in [[Stülke]] lab | ||
+ | ** pGP66 ([[pAC7]]), available in [[Stülke]] lab | ||
+ | ** pGP606 (mutant terminator, [[pAC6]]), available in [[Stülke]] lab | ||
+ | ** pGP532 ([[pAC7]]), available in [[Stülke]] lab | ||
+ | ** series of promoter deletions are available in [[pAC5]] and [[pAC6]], available in [[Stülke]] lab | ||
+ | ** series of RAT mutants are available in [[pAC6]], available in [[Stülke]] lab | ||
* '''GFP fusion:''' | * '''GFP fusion:''' |
Revision as of 15:50, 2 September 2009
- Description: trigger enzyme: major glucose permease of the PTS, EIICBA(Glc) and control of GlcT activity
Gene name | ptsG |
Synonyms | ptsX, crr |
Essential | no |
Product | trigger enzyme: glucose-specific enzyme IICBA component |
Function | glucose transport and phosphorylation, control of GlcT activity |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
MW, pI | 75,3 kDa, 5.40 |
Gene length, protein length | 2097 bp, 699 amino acids |
Immediate neighbours | glcT, ptsH |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU13890
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transport and phosphorylation of glucose, receives a phosphate from HPr at the IIA domain (His-620), the phosphate group is then transferred to the IIB domain (Cys-461) an finally to the incoming glucose. In the absence of glucose, PtsG phosphorylates and thereby inactivates the transcriptional antiterminator GlcT.
- Protein family: PTS permease, glucose permease (Glc) family PubMed, PTS enzyme II, glucose family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- 11x transmembrane domain (16–36, 89–109, 139–159, 180–200, 233–253, 283–303, 313–333, 338–358, 365–385, 388–408)
- PTS EIIC domain ( 1-424)
- PTS EIIB domain (439–520)
- PTS EIIA domain (568–672)
- Modification: transient phosphorylation (HPr-dependent) on His-620, then internal phosphotransfer from His-620 to Cys-461
- Cofactor(s):
- Effectors of protein activity:
- Localization: membrane protein PubMed
Database entries
- UniProt: P20166
- KEGG entry: [3]
- E.C. number: 2.7.1.69 9 2.7.1.69]
Additional information
Expression and regulation
- Regulation: expression activated by glucose (32 fold) PubMed
- Regulatory mechanism: transcriptional antitermination via the GlcT-dependent RNA-switch PubMed
- Additional information:
Biological materials
- Mutant: GP474 (cat), QB5436 (spc), QB5445 (erm), available in Stülke lab
- Expression vector:
- lacZ fusion:
- pGP34 (pAC5), available in Stülke lab
- pGP66 (pAC7), available in Stülke lab
- pGP606 (mutant terminator, pAC6), available in Stülke lab
- pGP532 (pAC7), available in Stülke lab
- series of promoter deletions are available in pAC5 and pAC6, available in Stülke lab
- series of RAT mutants are available in pAC6, available in Stülke lab
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References