Difference between revisions of "FusA"
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* '''Mutant:''' | * '''Mutant:''' | ||
− | * '''Expression vector:''' | + | * '''Expression vector:''' |
− | + | ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP840, available in [[Stülke]] lab | |
+ | ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pHW844]]: pGP848, available in [[Stülke]] lab | ||
+ | |||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
Revision as of 17:59, 1 September 2009
- Description: elongation factor G
Gene name | fusA |
Synonyms | fus |
Essential | yes PubMed |
Product | elongation factor G |
Function | translation |
MW, pI | 76 kDa, 4.615 |
Gene length, protein length | 2076 bp, 692 aa |
Immediate neighbours | rpsG, tufA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU01120
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: hydrolyses GTP
- Protein family: EF-G/EF-2 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) PubMed, PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P80868
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References