PcrA

• Description: ATP-dependent DNA helicase
  
  

Gene name	pcrA
Synonyms	yerF
Essential	yes PubMed
Product	ATP-dependent DNA helicase
Function	plasmid rolling-circle replication
MW, pI	83 kDa, 5.767
Gene length, protein length	2217 bp, 739 aa
Immediate neighbours	pcrB, ligA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YxaL-PcrA PubMed, YerB-PcrA PubMed, YwhK-PcrA PubMed

• Localization:

Database entries

• Structure: 2PJR (�product complex�, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (�substrate complex�, complex with DNA, Geobacillus stearothermophilus)

• UniProt: O34580

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed when no DNA damage is present (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

M-F Noirot-Gros, P Soultanas, D B Wigley, S D Ehrlich, P Noirot, M-A Petit
The beta-propeller protein YxaL increases the processivity of the PcrA helicase.
Mol Genet Genomics: 2002, 267(3);391-400
[PubMed:12073041] [WorldCat.org] [DOI] (P p)

M A Petit, E Dervyn, M Rose, K D Entian, S McGovern, S D Ehrlich, C Bruand
PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication.
Mol Microbiol: 1998, 29(1);261-73
[PubMed:9701819] [WorldCat.org] [DOI] (P p)