PlsX

• Description: acyl-acyl carrier protein (ACP):phosphate acyltransferase
  
  

Gene name	plsX
Synonyms	ylpD
Essential	yes PubMed
Product	acyl-acyl carrier protein (ACP):phosphate acyltransferase
Function	biosynthesis of phospholipids
MW, pI	35 kDa, 5.492
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	fapR, fabD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15890

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: plsX family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Interaction with PlsY PubMed

• Localization:

Database entries

• Structure: 1VI1

• UniProt: P71018

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed unless the cells experience a lack of malonyl-CoA (FapR) PubMed

• Regulatory mechanism:
  • FapR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed

• Antibody:

References

Yoshinori Hara, Masahide Seki, Satoshi Matsuoka, Hiroshi Hara, Atsushi Yamashita, Kouji Matsumoto
Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis.
Genes Genet Syst: 2008, 83(6);433-42
[PubMed:19282621] [WorldCat.org] [DOI] (P p)

Mika Yoshimura, Taku Oshima, Naotake Ogasawara
Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli.
BMC Microbiol: 2007, 7;69
[PubMed:17645809] [WorldCat.org] [DOI] (I e)

Luciana Paoletti, Ying-Jie Lu, Gustavo E Schujman, Diego de Mendoza, Charles O Rock
Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis.
J Bacteriol: 2007, 189(16);5816-24
[PubMed:17557823] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Luciana Paoletti, Alan D Grossman, Diego de Mendoza
FapR, a bacterial transcription factor involved in global regulation of membrane lipid biosynthesis.
Dev Cell: 2003, 4(5);663-72
[PubMed:12737802] [WorldCat.org] [DOI] (P p)

H R Morbidoni, D de Mendoza, J E Cronan
Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.
J Bacteriol: 1996, 178(16);4794-800
[PubMed:8759840] [WorldCat.org] [DOI] (P p)