Difference between revisions of "Crh"
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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2AK7 2AK7] (dimeric Crh-Ser46-P), [http://www.rcsb.org/pdb/explore.do?structureId=1ZVV 1ZVV] (CcpA-Crh-DNA complex), [http://www.rcsb.org/pdb/explore.do?structureId=2RLZ 2RLZ] (dimer), [http://www.rcsb.org/pdb/explore.do?structureId=1MU4 1MU4], [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=24634 NCBI], dimer [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=64943 NCBI], CcpA-Crh-DNA complex [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI], dimeric phosphor-Crh [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39567 NCBI] | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2AK7 2AK7] (dimeric Crh-Ser46-P), [http://www.rcsb.org/pdb/explore.do?structureId=1ZVV 1ZVV] (CcpA-Crh-DNA complex), [http://www.rcsb.org/pdb/explore.do?structureId=2RLZ 2RLZ] (dimer), [http://www.rcsb.org/pdb/explore.do?structureId=1MU4 1MU4], [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=24634 NCBI], dimer [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=64943 NCBI], CcpA-Crh-DNA complex [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI], dimeric phosphor-Crh [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39567 NCBI] | ||
| − | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/O06976 O06976] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34740] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34740] | ||
Revision as of 14:41, 20 July 2009
| Gene name | crh |
| Synonyms | yvcM |
| Essential | no |
| Product | catabolite repression HPr-like protein |
| Function | catabolite repression |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 9,2 kDa, 4.70 |
| Gene length, protein length | 255 bp, 85 amino acids |
| Immediate neighbours | yvcL, yvcN |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU34740
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Paralogous protein(s): HPr
Extended information on the protein
- Kinetic information:
- Domains: HPr domain (1–85)
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 2AK7 (dimeric Crh-Ser46-P), 1ZVV (CcpA-Crh-DNA complex), 2RLZ (dimer), 1MU4, NCBI, dimer NCBI, CcpA-Crh-DNA complex NCBI, dimeric phosphor-Crh NCBI
- UniProt: O06976
- KEGG entry: [2]
Additional information
Crh does not possess the phosphorylation site used for PTS phosphotransfer (His-15 in PtsH), it can only be phosphorylated on Ser-46
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information: Crh is weakly expressed. This results in part from a poorly conserved ribosomal binding site of the mRNA. PubMed
Biological materials
- Mutant: GP860 (aphA3), QB7097 (spc), available in Stülke lab
- Expression vector: pGP41 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), pGP734 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab
- lacZ fusion: see yvcI
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody: available in Stülke lab (not very good)
Labs working on this gene/protein
Boris Görke, University of Göttingen, Germany Homepage
Anne Galinier, University of Marseille, France
Wolfgang Hillen, Erlangen University, Germany Homepage
Richard Brennan, Houston, Texas, USA Homepage
Your additional remarks
References
