Difference between revisions of "Icd"
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* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1HQS 1HQS] | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1HQS 1HQS] | ||
− | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/P39126 P39126] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29130] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29130] |
Revision as of 14:04, 20 July 2009
- Description: isocitrate dehydrogenase
Gene name | icd |
Synonyms | citC |
Essential | no |
Product | isocitrate dehydrogenase |
Function | TCA cycle |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 46 kDa, 4.833 |
Gene length, protein length | 1269 bp, 423 aa |
Immediate neighbours | citZ, mdh |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU29130
Phenotypes of a mutant
reduced ability to sporulate PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH (according to Swiss-Prot)
- Protein family: isocitrate and isopropylmalate dehydrogenases family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten PubMed
- Domains:
- Cofactor(s): Mg2+, Mn2+
- Effectors of protein activity:
- Interactions: homodimer PubMed
- Localization: attached to the membrane PubMed
Database entries
- Structure: 1HQS
- UniProt: P39126
- KEGG entry: [3]
- E.C. number: 1.1.1.42
Additional information
This enzyme requires NADP+ exclusively. No activity was seen on the presence on NAD+ PubMed
Expression and regulation
- Sigma factor: SigA
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP666 (spc), GP672 (erm), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody: available in Linc Sonenshein lab
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Your additional remarks
References