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| − | * '''Description:''' enolase, glycolytic/ gluconeogenic enzyme<br/><br/>
| + | Testing, 1, 2, 3, 5 |
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''eno''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || enolase
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46,4 kDa, 4.49
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1290 bp, 430 amino acids
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pgm]]'', ''[[yvgK]]''
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15395]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:eno_context.gif]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |}
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| − | __TOC__
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| − | <br/><br/><br/><br/><br/><br/>
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Locus tag:''' BSU33900
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| − | ===Phenotypes of a mutant ===
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| − | * Essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10899]
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| − | === Additional information===
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| − | =The protein=
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:''' 2-phospho-D-glycerate = phosphoenolpyruvate + H<sub>2</sub>O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
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| − | * '''Protein family:''' enolase family (according to Swiss-Prot)
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| − | * '''Paralogous protein(s):'''
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
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| − | * '''Domains:'''
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| − | ** substrate binding domain (366–369)
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| − | * '''Modification:''' phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
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| − | * '''Cofactor(s):''' Mg2+
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| − | * '''Effectors of protein activity:'''
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| − | ** Inhibited by EDTA [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
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| − | * '''Interactions:''' [[Eno]]-[[PfkA]], [[Eno]]-[[Rny]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]
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| − | * '''Localization:''' cytoplasm (according to Swiss-Prot), cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] and membrane associated [http://www.ncbi.nlm.nih.gov/sites/entrez/18763711 PubMed]
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| − | === Database entries ===
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| − | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3ES8 3ES8] (from ''Oceanobacillus iheyensis'', complex with Mg(2+) and malate)
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| − | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37869 P37869]
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33900]
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| − | * '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.11 4.2.1.11]
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| − | === Additional information===
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| − | There are indications that this enzyme is an octamer [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
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| − | =Expression and regulation=
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| − | * '''Operon:'''
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| − | ** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]''
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| − | ** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]''
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| − | * '''Sigma factor:''' [[SigA]]
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| − | * '''Regulation:''' expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
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| − | **''[[cggR]]'': neg. regulated by [[CggR]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed], induced by sugar
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| − | **''[[pgk]]'': constitutive [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]
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| − | * '''Regulatory mechanism:''' transcription repression by [[CggR]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]
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| − | * '''Additional information:'''
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| − | =Biological materials =
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| − | * '''Mutant:'''
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| − | * '''Expression vector:''' pGP563 (N-terminal His-tag, in [[pWH844]]), pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:''' pHT315-yfp-eno, available in [[Mijakovic]] lab
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| − | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| − | * '''Antibody:''' available in [[Stülke]] lab
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| − | =Labs working on this gene/protein=
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| − | [[Stülke|Jörg Stülke]], University of Göttingen, Germany
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| − | [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| − | =Your additional remarks=
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| − | =References=
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| − | <pubmed> 17726680, 17218307, 12850135 , 19193632 , 11489127, 8021172, 17505547, 25885 </pubmed>
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