YacO

• Description: putative 23S rRNA methyltransferase
  
  

Gene name	yacO
Synonyms
Essential	no
Product	putative 23S rRNA methyltransferase
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	27 kDa, 8.633
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	mrnC, yacP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RNA methyltransferase trmH family (according to Swiss-Prot)

• Paralogous protein(s): YsgA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: Q06753

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Sigma factor: SigA PubMed

• Regulation: repression by cysteine (T-box) PubMed

• Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)