Difference between revisions of "LicT"

From SubtiWiki
Jump to: navigation, search
Line 90: Line 90:
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39805 P39805]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39805 P39805]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU39080]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39080]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 02:58, 25 June 2009

  • Description: transcriptional antiterminator of the bglPH operon

Gene name licT
Synonyms
Essential no
Product transcriptional antiterminator (BglG family)
Function required for substrate-dependent induction of bglPH
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 32 kDa, 5.944
Gene length, protein length 831 bp, 277 aa
Immediate neighbours bglS, yxiP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LicT context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU39080

Phenotypes of a mutant

no expression of the bglP-bglH operon

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binding to the mRNAs of bglS and the bglP-bglH operon, causes transcription antitermination (in presence of salicin and absence of glucose)
  • Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal RNA binding domain Pubmed
    • 2xPRD (PTS regulation domains) PubMed
  • Modification:
    • phosphorylation at His-100 in PRD-1 by phosphorylated BglP, inhibits LicT antitermination activity
    • phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-HPr, stimulates LicT antitermination activity
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure: 1L1C (complex with RAT), 1TLV (PRDs)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Josef Deutscher, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Michael Hecker, Greifswald, Germany Homepage

Your additional remarks

References

Original description

K Schnetz, J Stülke, S Gertz, S Krüger, M Krieg, M Hecker, B Rak
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family.
J Bacteriol: 1996, 178(7);1971-9
[PubMed:8606172] [WorldCat.org] [DOI] (P p)


Control of LicT activity


Structural analysis of LicT


LicT-RNA interaction