RnhC - Revision history

Fabian Commichau: /* Biological materials */

2014-10-07T13:31:00Z

‎Biological materials

Jstuelk: /* Reviews */

2014-04-24T11:59:53Z

‎Reviews

134.76.38.147 at 14:27, 2 April 2014

2014-04-02T14:27:40Z

Fabian Commichau: /* Biological materials */

2013-10-29T15:37:02Z

‎Biological materials

Fabian Commichau: /* Biological materials */

2013-10-29T15:35:58Z

‎Biological materials

Jstuelk at 15:46, 25 July 2013

2013-07-25T15:46:10Z

134.76.70.252 at 14:13, 16 May 2013

2013-05-16T14:13:17Z

134.76.70.252 at 10:57, 14 May 2013

2013-05-14T10:57:06Z

134.76.70.252 at 13:32, 13 May 2013

2013-05-13T13:32:15Z

134.76.70.252 at 14:41, 7 August 2012

2012-08-07T14:41:46Z

← Older revision		Revision as of 13:31, 7 October 2014
Line 1:		Line 1:
−	* '''Description:~~''' [[RNase]] HIII, endoribonuclease <br/><br/>~~	+	{{#bank: RnhC}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''rnhC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ysgB ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| Mg2+-dependent [[RNase]] HIII~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| endonucleolytic cleavage of RNA <br/>in RNA-DNA hybrid molecules~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28620 rnhC]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 33 kDa, 10.07~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 939 bp, 313 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[zapA]]'', ''[[pheT]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28620 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28620 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28620 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:rnhC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnhC_2926031_2926972_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:rnhC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28620]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[Rnases]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU28620
−
−	~~===Phenotypes of a mutant ===~~
−	* a ''[[rnhB]] [[rnhC]]'' double mutant grows poorly {{PubMed\|23882084}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28620&redirect=T BSU28620]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnhC.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12324]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
−
−	* '''Protein family:''' RnhC subfamily (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** RnhC interacts with the [[RNA polymerase]] {{PubMed\|21710567}}
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28620&redirect=T BSU28620]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2D0B 2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), [http://www.rcsb.org/pdb/explore.do?structureId=2D0A 2D0A] (Geobacillus stearothermophilus, 47% identity)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P94541 P94541]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28620]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[rnhC]]'' {{PubMed\|8969504}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnhC_2926031_2926972_1 rnhC] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' BP430 (''ΔrnhC::cat'') available in [[Fabian Commichau]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>19228197 24464998 </pubmed>~~
−
−	~~==Original publications==~~
−	~~<pubmed>8969504 10094689 9888800 17905985 23882084 21710567</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 135: / Line 135: @@
 =References=
 ==Reviews==
-<pubmed>19228197 </pubmed>
+<pubmed>19228197 24464998 </pubmed>
 ==Original publications==
 <pubmed>8969504 10094689  9888800 17905985 23882084 21710567</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 53: / Line 53: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnhC.html]
@@ Line 88: / Line 89: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2D0B 2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity),  [http://www.rcsb.org/pdb/explore.do?structureId=2D0A 2D0A] (Geobacillus stearothermophilus, 47% identity)

@@ Line 115: / Line 115: @@
 =Biological materials =
-* '''Mutant:''' BP430 (''ΔrnhC::cat'') available in
+* '''Mutant:''' BP430 (''ΔrnhC::cat'') available in [[Fabian Commichau]]'s lab
 * '''Expression vector:'''

@@ Line 115: / Line 115: @@
 =Biological materials =
-* '''Mutant:'''
+* '''Mutant:''' BP430 (''ΔrnhC::cat'') available in
 * '''Expression vector:'''

@@ Line 35: / Line 35: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 54: / Line 50: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 138: / Line 135: @@
 <pubmed>19228197 </pubmed>
 ==Original publications==
-'''Additional publications:''' {{PubMed|21710567}}
+<pubmed>8969504 10094689  9888800 17905985 23882084 21710567</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnhC_2926031_2926972_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rnhC_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnhC_2926031_2926972_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rnhC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28620]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[zapA]]'', ''[[pheT]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28620 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28620 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28620 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28620 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28620 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28620 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:rnhC_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || endonucleolytic cleavage of RNA <br/>in RNA-DNA hybrid molecules
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU28620 rnhC]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28620 rnhC]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 10.07
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[zapA]]'', ''[[pheT]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14822&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28620 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28620 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28620 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:rnhC_context.gif]]